Walther Lab Research
Human cells go to great lengths to protect the integrity of the genetic information located on DNA chromosomes. When chromosomes of a cell are damaged, one or more genes found on those chromosomes may no longer function normally and the resulting cell may lose the ability to carry out important functions required for the organism to thrive and survive. Luckily cells have multiple repair pathways able to recognize and fix damaged DNA. Failure to do so can lead to genetic disorders, cancer, and even cell death.
It
can be difficult to study chromosomal maintenance pathways in human
cells, so other organisms have been used as model systems to understand
these processes. The budding yeast Saccharomyces cerevisiae, which
is
commonly
referred
to as Baker’s yeast (used in bread and beer–making),
is a unicellular organism that shows a high level of similarity in its
intracellular processes to the intracellular processes in human
cells. Often, the same proteins are found in both human and
yeast cells and these proteins carry out the same functions in human
and yeast cells. Much of our current understanding of human
intracellular processes has come from studies in Baker’s yeast.
Most
DNA repair pathways require a protein called Replication Protein A
(RPA). RPA is an evolutionarily conserved, single-stranded DNA
binding protein that is found in both humans and yeast. In
addition to playing a role in DNA repair, RPA is involved in DNA
replication, telomere maintenance, cell-cycle control, and
transcription. In the lab, we are using a combination of
molecular biology, molecular genetics, biochemistry, and cell biology
to better understand the role of RPA in these different
processes. A better understanding of RPA’s roles in these
processes will give us insights into how chromosomal integrity is
maintained in cells, and may provide new targets to treat diseases such
as cancer.